Stereochemistry of 2-phenylethylamine oxidation catalyzed by bacterial copper amine oxidase.
نویسندگان
چکیده
The stereochemical course of the reaction catalyzed by a copper amine oxidase from Arthrobacter globiformis has been investigated using 2-phenylethylamine stereospecifically deuterium-labeled at the C1 position. Measurements of deuterium content in the product, phenylacetaldehyde, by gas chromatography-mass spectrometry revealed stereospecific abstraction of the pro-S hydrogen during the enzymatic oxidation, as predicted from the structure modeling for the enzyme-bound substrate.
منابع مشابه
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ورودعنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 67 12 شماره
صفحات -
تاریخ انتشار 2003